Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Please note that all translations are automatically generated. Click here for the English version.

3.0K Views

•

11:04 min

•

September 3rd, 2020

DOI :

10.3791/61493-v

September 3rd, 2020

•

Carlos-Camilo Silva*¹^,², Montserrat Bolaños-Hurtado*¹, Cinthia Juárez-Tapia⁴, Angélica Flores¹^,², Isabel Arrieta-Cruz³^,⁵, María-Esther Cruz³, Roberto Domínguez¹^,²^,³

¹Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ²Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ³Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ⁴Facultad de Ciencias, Universidad Nacional Autónoma de México, ⁵Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health

文字起こし

要約

さらに動画を探す

Ovulation Regulation

Reversible Inactivation

Microinjections

Physiological Processes

この動画の章

0:04

Introduction

0:47

Construction of Bilateral Cannulas, Obturators, and Caps

2:49

Construction of Microinjectors

4:10

Animal Maintenance and Vaginal Smears

5:31

Microinjection of TTX or Vehicle Solutions into Freely Moving Rats

7:27

Tissue Processing

8:44

Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)

10:08

Conclusion

関連動画

Copyright © 2023 MyJoVE Corporation. All rights reserved