Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
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11:04 min
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September 3rd, 2020
DOI :
September 3rd, 2020
•Carlos-Camilo Silva*1,2, Montserrat Bolaños-Hurtado*1, Cinthia Juárez-Tapia4, Angélica Flores1,2, Isabel Arrieta-Cruz3,5, María-Esther Cruz3, Roberto Domínguez1,2,3
1Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 2Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 3Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 4Facultad de Ciencias, Universidad Nacional Autónoma de México, 5Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health
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0:04
Introduction
0:47
Construction of Bilateral Cannulas, Obturators, and Caps
2:49
Construction of Microinjectors
4:10
Animal Maintenance and Vaginal Smears
5:31
Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
7:27
Tissue Processing
8:44
Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)
10:08
Conclusion
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