Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

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3.0K Views

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11:04 min

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September 3rd, 2020

DOI :

10.3791/61493-v

September 3rd, 2020

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Carlos-Camilo Silva*¹^,², Montserrat Bolaños-Hurtado*¹, Cinthia Juárez-Tapia⁴, Angélica Flores¹^,², Isabel Arrieta-Cruz³^,⁵, María-Esther Cruz³, Roberto Domínguez¹^,²^,³

¹Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ²Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ³Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, ⁴Facultad de Ciencias, Universidad Nacional Autónoma de México, ⁵Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health

Transkript

Özet

Daha Fazla Video Keşfet

Ovulation Regulation

Reversible Inactivation

Microinjections

Physiological Processes

Bu videodaki bölümler

0:04

Introduction

0:47

Construction of Bilateral Cannulas, Obturators, and Caps

2:49

Construction of Microinjectors

4:10

Animal Maintenance and Vaginal Smears

5:31

Microinjection of TTX or Vehicle Solutions into Freely Moving Rats

7:27

Tissue Processing

8:44

Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)

10:08

Conclusion

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