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JoVE Journal
Biology
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Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
16.5K Views
•
11:28 min
•
March 31st, 2012
DOI :
March 31st, 2012
•Chapters in this video
0:05
Title
1:46
Transformation of E. coli with the pGEX-RhoA(G17A) Construct
2:55
Preparation of GST-RhoA(G17A) Beads
7:50
GEF Pulldown Assay with Nucleotide-free RhoA(G17A) Beads
10:11
Representative Pull Down Results
11:03
Conclusion
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